Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton using A0004-70D. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and vesicle/ cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488